Multiparameter Flow-Cytometric Analysis ofbcl-2 and Fas Expression in Normal and Neoplastic Hematopoiesis

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Abstract
Apoptosis (programmed cell death) is an important regulatory mechanism in hematopoiesis, and is thought to be a principal mechanism of action of cytotoxic chemotherapy. Proteins that modulate apoptosis include bcl-2, which inhibits apoptosis, and Fas (CD9S, also known as APO-1), which induces apoptosis in susceptible cells bound by Fas ligand (FasL). To characterize precisely the expression of these apoptosis-regulatory proteins in normal and neoplastic hematopoiesis, the authors have performed multiparameter flow cytometric (FCM) analysis in a series of normal and abnormal marrow specimens. Among normal hematopoietic elements, bcl-2 expression was highest in myeloblasts (29 [± 9] × 103 molecules of equivalent soluble fluorochrome [MESF]), and lymphocytes (28 [± 7] × 103 MESF). bcl-2 was essentially undetectable in granulocytes and nucleated red blood cells, whereas monocytes and B-cell precursors expressed intermediate levels of bcl-2 (11 [± 4] × 103 and 7 [± 1] × 103 MESF, respectively). Fas expression increased with granulocytic and monocytic differentiation; myeloblasts expressed 8 [± 2] × 103 MESF, whereas granulocytes (15 [± 2] × 103 MESF) and monocytes (28 [± 5] × 103 MESF) displayed relatively greater intensity of staining for Fas. Among lymphoid cells, Fas expression was heterogeneous. B cells expressed lower intensity Fas staining than both CD4+ and CD8+ T cells. Myeloblasts in 30 cases of myeloid leukemia and myelodysplasia studied for bcl-2 and/or Fas expression manifested variable levels of these molecules (range 9–105 × 103 MESF for bcl-2 and 3-33 × 103 MESF for Fas). In addition, intraclonal heterogeneity of bcl-2 and Fas expression was seen in certain cases of AML, which correlated with extent of differentiation. Among 28 cases of B-precursor ALL studied for bcl-2 and/or Fas expression, bcl-2 ranged from 22 to 60 × 103 MESF (P <.001 versus normal marrow B-cell precursors), and Fas varied between essentially undetectable levels and 6 × 103 MESF. In summary, normal marrow subsets display characteristic levels of the apoptosis-regulatory molecules, bcl-2 and Fas. In hematopoietic neoplasms, expression of bcl-2 and Fas varies among cases, and in some instances, within leukemic blast populations. Further study is required to understand the potential significance of this heterogeneous expression of bcl-2 and Fas in hematologic neoplasia.

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