Cryofracturing as a Technique for the Study of Fungal Structures in the Scanning Electron Microscope

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Abstract
When freeze-fractured fungal structures are critical point dried and then examined in the scanning electron microscope a number of structures are evident that are obscured by other techniques. A comparison of freeze-fracture to the techniques of razor blade sectioning and microtomy of wax-embedded or plastic-embedded material was carried out or reviewed from the literature. In most cases superior results can be obtained with the cryofracture technique followed by critical point drying.