Asexual Embryogenesis in Theobroma cacao L.1

Abstract
Cultured immature sexual embryos of Theobroma cacao proliferated asexual embryos, but embryogenesis was not observed from leaf, pericarp, or ovule tissues. Cotyledons from immature, unpigmented embryos (2.5 to 10 mm long) were highly embryognie, while larger more mature, pigmented cotyledons initiated roots as their only form of organized development. Embryogenesis occurred in light and darkness when a basal medium was supplemented with coconut water and 1.5 mg/liter NAA. Proliferated embryos recultured in a liquid medium continued development.

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