Biochemical characterization of broad-specificity enzymes using multivariate experimental design and a colorimetric microplate assay: characterization of the haloalkane dehalogenase mutants
- 1 March 2001
- journal article
- research article
- Published by Elsevier in Journal of Microbiological Methods
- Vol. 44 (2) , 149-157
- https://doi.org/10.1016/s0167-7012(00)00250-5
Abstract
No abstract availableKeywords
Funding Information
- Ministerstvo Školství, Mládeže a Tělovýchovy (J07/98:143100005, LN00A016)
This publication has 13 references indexed in Scilit:
- Analysis of the reaction mechanism and substrate specificity of haloalkane dehalogenases by sequential and structural comparisons.Protein Engineering, Design and Selection, 1999
- Construction and Characterization of Histidine-Tagged Haloalkane Dehalogenase (LinB) of a New Substrate Class from a γ-Hexachlorocyclohexane-Degrading Bacterium, Sphingomonas paucimobilis UT26Protein Expression and Purification, 1999
- Dehalogenases applied to industrial-scale biocatalysisCurrent Opinion in Biotechnology, 1999
- Haloalkane Dehalogenases: Steady-State Kinetics and Halide InhibitionBiochemistry, 1999
- A colorimetric assay for detecting haloalkane dehalogenase activityJournal of Microbiological Methods, 1998
- Kinetic Characterization and X-ray Structure of a Mutant of Haloalkane Dehalogenase with Higher Catalytic Activity and Modified Substrate Range,Biochemistry, 1996
- Histidine 289 Is Essential for Hydrolysis of the Alkyl-enzyme Intermediate of Haloalkane DehalogenaseJournal of Biological Chemistry, 1995
- Activation of an Asp‐124→Asn mutant of haloalkane dehalogenase by hydrolytic deamidation of asparagineFEBS Letters, 1995
- Site-Directed Mutagenesis and Oxygen Isotope Incorporation Studies of the Nucleophilic Aspartate of Haloalkane DehalogenaseBiochemistry, 1994
- Principal component analysisChemometrics and Intelligent Laboratory Systems, 1987