The respiratory response to heat shock in Neurospora crassa

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Abstract
A sharp decrease in oxygen uptake occurred in Neurospora crassa cells that were transferred from 30°C to 45°C, and the respiration that resumed later at 45°C was cyanide-insensitive. Energization of mitochondria, measured in vivo with fluorescence microscopy and a carbocyanine dye, also declined sharply in cells at 45°C. Electron microscopy showed no changes in mitochondrial complexity; however, the cytoplasm of heat-shocked cells was deficient in glycogen granules.

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