Formation of Inosine-5'-Monophosphate by a Kinase in Cell-Free Extracts of Ehrlich Ascites Cells in Vitro
- 1 February 1968
- journal article
- research article
- Published by Frontiers Media SA in Experimental Biology and Medicine
- Vol. 127 (2) , 432-440
- https://doi.org/10.3181/00379727-127-32709
Abstract
Cell-free extracts from Ehrlich ascites tumor cells were found to convert inosine to inosine 5′-monophosphate at a rate comparable to the conversion of adenosine to adenosine 5′-monophosphate by the same extracts. This inosine kinase appeared to be distinct from adenosine kinase. Conditions necessary for optimum activity were studied. Magnesium ions were required, as was a relatively low level of ATP and an ATP-regenerating system. The pH optimum was approximately 7.4.This publication has 15 references indexed in Scilit:
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