AN ANALYSIS OF FOURTH DERIVATIVE SPECTRA
- 1 December 1970
- journal article
- Published by Wiley in Photochemistry and Photobiology
- Vol. 12 (6) , 451-456
- https://doi.org/10.1111/j.1751-1097.1970.tb06077.x
Abstract
Abstract— –Fourth derivative spectra of chloroplast at – 196°C, obtained by four sequential differentiations of the absorption spectrum, were examined to determine the conditions for optimal signal‐to‐noise ratio and resolution. An appreciable improvement in the signal‐to‐noise ratio was found when the four differentiating intervals were nearly but not exactly equal. The process of differentiating digitized data with the computer was explored in order to define the rules for optimization of the fourth derivative curves.Keywords
This publication has 1 reference indexed in Scilit:
- HIGHER DERIVATIVE ANALYSIS OF COMPLEX ABSORPTION SPECTRAPhotochemistry and Photobiology, 1970