Ionic strength effects on the stability and conformation of Penlctllium chrysogenum mycophage double stranded RNA
Open Access
- 1 June 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (6) , 759-772
- https://doi.org/10.1093/nar/2.6.759
Abstract
The effect of [NR+] on the stability and conformation of penicillium chrysogenum mycophage dsRNA (PCMdsRNA) was investigated using CD and UV optical techniques. Thermal melting profiles reveal prominent fine structure attributed to at least four regions of structural dissimilarity. A constant increased thermal stability of the dsRNA compared to DNA of the same base composition was observed over a oncentration range of 1.5 × 10−4 M to 4.5 × 10−2 M Na+. At low ionic strengths ([Na+]< 10−3 M) Tm becomes independent of further decreases in [Na+] unless the dsRNA is exposed to high concentrations of EDTA, suggesting the involvement of tightly bound divalent cation. At relatively high ionic strengths ([Na+] > 0.1 M) a postulated A – A' … conformation change occurs.Keywords
This publication has 23 references indexed in Scilit:
- Viral Infection and Host DefenseScience, 1974
- Fractionation of nucleic acids from Penicillium chrysogenum and associated ribonucleic acid viruses by selective exclusion and retention in agarose gelsBiochemical Journal, 1974
- Fungal viruses.1974
- Conformational studies of reovirus single-stranded RNAs synthesized in vitroBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Refinement of the structure of B-DNA and implications for the analysis of X-ray diffraction data from fibers of biopolymersJournal of Molecular Biology, 1973
- Viruses with Double-stranded RNA GenomesJournal of General Virology, 1973
- Binding of ethidium bromide to double-stranded ribonucleic acidBiochemistry, 1973
- Properties of viruslike particles of Penicillium chrysogenum: One double-stranded RNA molecule per particleVirology, 1972
- The geometry of nucleic acidsProgress in Biophysics and Molecular Biology, 1970
- X-Ray Diffraction Investigations of Complementary RNANature, 1964