Immunochemical Characterization of Pyruvate Dehydrogenase Complex in Rat Brain
- 1 February 1985
- journal article
- research article
- Published by Wiley in Journal of Neurochemistry
- Vol. 44 (2) , 593-599
- https://doi.org/10.1111/j.1471-4159.1985.tb05453.x
Abstract
Pyruvate dehydrogenase complex (PDHC) in rat brain was studied immunochemically, using antibodies against the bovine kidney PDHC, by immunoblotting, immunoprecipitation, inhibition of enzyme activity, and enzyme-linked immunoabsorbent assay (ELISA). The immunoblots showed that the antibodies bound strongly to the alpha peptide of the pyruvate dehydrogenase (E1) component, and to the dihydrolipoyl transacetylase (E2) and the dihydrolipoyl dehydrogenase (E3) components of PDHC. A similar immunoblotting pattern was observed in all eight brain regions examined. On immunoblotting of the subcellular fractions, these PDHC peptides were observed in mitochondria and synaptosomes but not in the postmitochondrial supernatants. This agrees with other evidence that brain PDHC is localized in the mitochondria. These results, together with those from sodium dodecyl sulfate-polyacrylamide gel electrophoresis of the immunoprecipitin, also showed that the alpha E1, beta E1, and E3 peptides of rat brain PDHC are very similar in sizes to those of the bovine kidney PDHC, being 42, 36, and 58 kD, respectively. The size of the E2 peptide, 66 kD, is different from that of bovine kidney E2, 73 kD. The relative abundance of PDHC protein in nonsynaptic mitochondria was compared by enzyme activity titration and ELISA. Both methods demonstrated that the amount of PDHC antigen in the mitochondria from cerebral cortex is greater than that in the olfactory bulb mitochondria. This is consistent with the results of the activity measurement. The ELISA also showed that the PDHCs in both mitochondrial populations are antigenically similar.(ABSTRACT TRUNCATED AT 250 WORDS)Keywords
This publication has 29 references indexed in Scilit:
- Studies on the Bovine Brain Pyruvate Dehydrogenase Complex Using the Antibodies Against Kidney Enzyme ComplexJournal of Neurochemistry, 1984
- Properties and Regional Distribution of Pyruvate Dehydrogenase Kinase in Rat BrainJournal of Neurochemistry, 1984
- Pyruvate Dehydrogenase Phosphate (PDHb) Phosphatase in Brain: Activity, Properties, and Subcellular LocalizationJournal of Neurochemistry, 1983
- Evidence that a 41,000 dalton brain phosphoprotein is pyruvate dehydrogenaseBiochemical and Biophysical Research Communications, 1980
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979
- THE CONTROL OF PYRUVATE DEHYDROGENASE IN ISOLATED BRAIN MITOCHONDRIAJournal of Neurochemistry, 1978
- PYRUVATE DEHYDROGENASE ACTIVATION IN RAT BRAIN CORTICAL SLICES BY ELEVATED CONCENTRATIONS OF EXTERNAL POTASSIUM IONSJournal of Neurochemistry, 1977
- PROVENANCE OF THE ACETYL GROUP OF ACETYLCHOLINE AND COMPARTMENTATION OF ACETYL‐CoA AND KREBS CYCLE INTERMEDIATES IN THE BRAIN IN VIVOJournal of Neurochemistry, 1974
- Interconversion and Kinetic Properties of Pyruvate Dehydrogenase from BrainHoppe-Seyler´s Zeitschrift Für Physiologische Chemie, 1971
- REGIONAL STUDIES OF CATECHOLAMINES IN THE RAT BRAIN‐IJournal of Neurochemistry, 1966