Partial purification of a low molecular weight inhibitor of epidermal DNA synthesis

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Abstract
An attempt was made to purify factors present in aqueous extracts of pig epidermis which inhibit epidermal cell proliferation. A lipophilic factor of low MW (< 10,000) was shown to inhibit DNA synthesis as measured by the uptake of [3H]thymidine in mouse ear epidermis. Purification by alcohol precipitation, ethyl acetate extraction and silicic acid column chromatography produced a 15-fold increase in the specific activity of the inhibitory action. Aggregation or absorption of this low MW factor may explain the high MW of epidermal cell proliferation inhibitors previously studied and the difficulty in their characterization.