Nuclear analogs of β-lactam antibiotics. VIII. Synthesis of 3-acetoxymethyl-Δ3-O-2-isocephems

Abstract
The synthesis of 7.beta.-(-2''-thienylacetamido)-3-acetoxymethyl-.DELTA.3-O-2-isocephem-4-carboxylic acid (17) is described. Treatment of enol mesylate or triflate with triethylamine gave the allene, which gave the diiodide upon treatment with iodine. The diiodide gave benzyl 7.beta.-azido-3-acetoxymethyl-.DELTA.3-O-2-isocephem-4-carboxylate (12) on treatment with potassium acetate. Hydrogenolysis of 12 gave the amino acid, which was converted to (17) upon treatment with 2-thienylacetyl chloride. Resolution of 17 into its optical antipodes was carried out. The antibiotic [antibacterial] activities of racemic 17 and each antipode is compared to that of cephalothin. Antibiotic activity was found to reside in the dextrorotatory isomer.

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