An Organ Culture Study of Frog Renal Tumor and its Effects on Normal Frog Kidney in vitro

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Abstract
Normal kidney tissues of the frog, Rana pipiens, were cultivated with pieces of frog renal tumor or exposed to tumor nitrates and maintained in a new ‘vertical’ type of organ culture. After several weeks, experimental cultures showed proliferative changes that fulfilled ordinary clinical histopathological criteria for malignant transformation. The temperature-associated, summer-winter cycle of herpes-type viral nuclear inclusion bodies observed in the Lucké tumor in nature [Rafferty, 1964] was duplicated in vitro. When tumor pieces with no viral inclusions were subjected to low temperatures in organ culture, inclusion bodies were induced; the same inclusion bodies disappeared after a few days’ culture at room temperature.

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