Protargol impregnation of plastic-embedded semi-thin sections: A simple method to select degenerative areas for electron microscopy
- 1 March 1977
- journal article
- research article
- Published by Springer Nature in Journal Of Neural Transmission-Parkinsons Disease and Dementia Section
- Vol. 40 (1) , 27-32
- https://doi.org/10.1007/bf01250278
Abstract
0.5μ, plastic-embedded sections, obtained from aldehyde-osmium fixed rat and cat spinal cord, were impregnated in a 0.5% Protargol solution for 24 hours at 56°C. Reduction was performed in a sodium sulphite-(5%) hydroquinone (1%) developer. Terminals undergoing Wallerian degeneration stand out as easily discernible black dots; corresponding osmiophilic degenerative patterns are demonstrated in consecutive thin electron microscopic sections. This simple technique enables successful trimming of blocks to obtain areas with the highest frequency of terminal degeneration.This publication has 6 references indexed in Scilit:
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