Escherichia coli dnaT gene function is required for pBR322 plasmid replication but not for R1 plasmid replication
- 31 May 1989
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 171 (6) , 2975-2980
- https://doi.org/10.1128/jb.171.6.2975-2980.1989
Abstract
Plasmid pBR322 was unable to replicate in a temperature-sensitive dnaT1 strain at a nonpermissive temperature, whereas a pBR322-derived plasmid carrying the wild-type dnaT+ gene was able to replicate under the same conditions. In contrast to pBR322, plasmid R1 could replicate in the dnaT1 strain at a nonpermissive temperature. In keeping with this finding, in vitro replication of plasmid R1 did not require DnaT protein.This publication has 32 references indexed in Scilit:
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