STIMULATORY EFFECTS OF PRODUCTS FROM INFLAMMATORY EXUDATES UPON STEM-CELL PRODUCTION

  • 1 January 1978
    • journal article
    • research article
    • Vol. 6  (4) , 405-409
Abstract
The present study is based on the hypothesis that a humoral agent produced in inflammatory exudates is transported to the bone marrow where it stimulates CFU-S [pluripotential stem cells] to differentiate into leukocytic precursors. Inflammatory exudates were produced by the s.c. implantation of a sterile acrylic cup filled with bacteria-free Hanks'' balanced salt solution (HBSS) into B6D2F1 mice. The cups were removed 24 h later, and the cells were separated from the supernatant. Cells collected from individual mice were pooled, as was the supernatant. Normal mice received (via tail vein) exudate cells, supernatant, plasma from cup-implanted or from normal mice, bone marrow cells or splenic cells from normal animals or HBSS. Another 24 h later bone marrow was removed from all mice and injected into previously lethally irradiated mice or suspended in vitro in agar-supported media. Nine days later the mice were euthanatized, the spleens removed and colonies (CFU-S) counted. At the same time, colonies (CFU-C [granulocytic progenitor cells]) were enumerated from the agar plates. The bone marrow obtained from mice stimulated with exudate cells or with supernatant or plasma obtained from cup-implanted mice probably contained a significantly greater number of CFU-S and CFU-C than did any 1 of the 4 control groups.