Thyrotropin‐releasing hormone‐induced depletion of Gqα/G11α proteins from detergent‐insensitive membrane domains
Open Access
- 20 December 1999
- journal article
- Published by Wiley in FEBS Letters
- Vol. 464 (1-2) , 35-40
- https://doi.org/10.1016/s0014-5793(99)01666-x
Abstract
The role of detergent‐insensitive membrane domains (DIMs) in desensitisation of the G protein‐coupled receptor‐mediated hormone response was studied in clone E2M11 of HEK293 cells which stably express high levels of both thyrotropin‐releasing hormone (TRH) receptors and G11α G protein. DIMs were prepared by flotation in equilibrium sucrose density gradients and characterised by a panel of membrane markers representing peripheral, glycosylphosphatidylinositol‐bound as well as integral membrane proteins (caveolin, CD29, CD55, CD59, CD147, the α subunit of Na,K‐ATPase) and enzyme activities (alkaline phosphatase, adenylyl cyclase). Caveolin‐containing DIMs represented only a small fraction of the overall pool of Gqα/G11α‐rich domains. Prolonged stimulation of E2M11 cells with TRH resulted in dramatic depletion of Gqα/G11α from all DIMs, which was paralleled by a concomitant Gqα/G11α increase in the high‐density gradient fractions containing the bulk‐phase membrane constituents soluble in 1% Triton X‐100. Distribution of membrane markers was unchanged under these conditions. Membrane domains thus represent a substantial structural determinant of the G protein pool relevant to desensitisation of hormone action.Keywords
This publication has 33 references indexed in Scilit:
- Overexpression of the G protein G11α prevents desensitization of CA2+ response to thyrotropin-releasing hormoneLife Sciences, 1999
- Visualization of distinct patterns of subcellular redistribution of the thyrotropin-releasing hormone receptor-1 and Gqα /G11α induced by agonist stimulationBiochemical Journal, 1999
- Agonist-induced Internalization of the G Protein G11α and Thyrotropin-releasing Hormone Receptors Proceed on Different Time ScalesPublished by Elsevier ,1998
- Organization of G Proteins and Adenylyl Cyclase at the Plasma MembraneMolecular Biology of the Cell, 1997
- Activation-induced subcellular redistribution of Gs alpha.Molecular Biology of the Cell, 1996
- Isoproterenol-induced subcellular redistribution of G-protein β subunits in S49 lymphoma cells demonstrated by a novel competitive ELISAArchives of Physiology and Biochemistry, 1995
- The Short and Long Forms of the alpha Subunit of the Stimulatory Guanine-Nucleotide-Binding Protein are Unequally Redistributed During (-)-Isoproterenol-Mediated Desensitization of Intact S49 Lymphoma CellsEuropean Journal of Biochemistry, 1994
- Agonist‐induced Transfer of the α Subunits of the Guanine‐nucleotide‐binding Regulatory Proteins Gq and G11, and of Muscarinic m1 Acetylcholine Receptors from Plasma Membranes to a Light‐vesicular Membrane FractionEuropean Journal of Biochemistry, 1994
- Plasma‐membrane‐independent pool of the α subunit of the stimulatory guanine‐nucleotide‐binding regulatory protein in a low‐density‐membrane fraction of S49 lymphoma cellsEuropean Journal of Biochemistry, 1992
- Stimulation of beta-adrenergic receptors of S49 lymphoma cells redistributes the alpha subunit of the stimulatory G protein between cytosol and membranes.Proceedings of the National Academy of Sciences, 1989