A Rapid Ammonium Sulfate Precipitation Technic for Separating Serum Vitamin B12-binding Proteins: Method and Applications
Open Access
- 1 September 1974
- journal article
- research article
- Published by Oxford University Press (OUP) in American Journal of Clinical Pathology
- Vol. 62 (3) , 367-372
- https://doi.org/10.1093/ajcp/62.3.367
Abstract
Current methods for separating serum vitamin B12-binding proteins suffer from one, or more frequently both, of two handicaps. They do not separate all three serum binders, and they are usually complex and time-consuming. Therefore, a simple, one-step (NH4)2SO4 precipitation technic derived from the serum polyfractionation method of Effersøe, is presented. The precipitate contains 100% of the serum transcobalamin (TC) II. The supernate contains 86-100 % of the TC I and the third binder. Fractionation is virtually identical to that obtained by the more laborious Sephadex gel chromatography. The precipitation technic does not isolate the third binder but, when combined with the equally simple DEAE-cellulose batch separation method, can be used for rapid estimation of the three binders in serum specimens. Results of a clinical survey of levels of the three serum binders in various hematologic disorders are also presented.Keywords
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