The use of a synthetic medium in the isolation of the somatic antigens of Bact. typhi-murium and Bact. typhosum

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Abstract
Chemical fractions of these organisms, free from contaminating substances such as agar from the media, can be obtained only when the organisms are grown in a liquid medium, preferably containing only inorganic salts and glucose. Antigenic fractions have been prepd. by tryptic digestion, extraction of the organisms with cold CCl2.COOH, and extraction with di-ethyleneglycol; the 3 products are essentially similar in composition and toxicity. A method for determining the galactose content of bacterial bodies and antigenic fractions has been elaborated and used for the determination of the extent of agar contamination of bacterial fractions obtained from agar media.

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