HETEROGENEITY OF MOLECULAR-SIZE OF FACTOR-VIII-VONWILLEBRAND FACTOR IN VONWILLEBRANDS DISEASE

  • 1 January 1981
    • journal article
    • research article
    • Vol. 45  (3) , 272-275
Abstract
Patterns of VIIIR:AG [factor VIII-related antigen] in the plasma and its fractions, cryoprecipitate and cryosupernatant, from various types of von Willebrand''s disease (vWd) were observed [in patients] by SDS [sodium dodecylsulfate] 1.5% polyacrylamide gel electrophoresis-crossed immunoelectrophoresis (SDS PAGE-CIE). VIIIR:AG in normal cryoprecipitate showed several precipitin peaks which correspond to MW ranging from 8 .times. 105 to 1 .times. 107 daltons and are similar to those in normal plasma. Normal cryosupernatant VIIIR:AG gave smaller MW from 8 .times. 105 to 2 .times. 106 daltons. VIIIR:AG in the plasma and cryoprecipitate from 2 patients with classical vWd gave low precipitin peaks with MW in the normal range. VIIIR:AG from 2 patients with subgroup A variant which showed fast anodal migration on the conventional CIE, presented 3 peaks with MW of 8 .times. 105 to 3 .times. 106 which are similar to those in normal cryosupernatant. VIIIR:AG from 2 patients with subgroup B variant which showed normal migration on the CIE, gave normal patterns through all fractions.

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