Hybridomas Secreting Monoclonal Antibody with Specificity for Toxoplasma gondii

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Abstract
The successful establishment of 8 functional hybridomas is reported between mouse myeloma cells and spleen cells from mice hyperimmune to T. gondii, as monitored by indirect immunofluorescence and radioimmunoassay techniques. Of these 8 cloned hybridomas, 2 showed positive reactivity in the Sabin Feldman dye test. Three hybridoma clones selected, following fusion of myeloma cells with freshly harvested spleen cells from hyperimmune mice, secreted antitoxoplasma antibody of the Ig[immunoglobulin]G class into the culture medium. When spleen cells from hyperimmune mice were precultured in vitro for 5 days in the presence of Con [concanavalin] A and LPS [lipopolysaccharide] and then fused with myeloma cells, 5 of the stable hybridomas secreted antitoxoplasma antibody of the IgM class, while 1 secreted antibody of the IgG class. These monoclonal antitoxoplasma antibodies should permit a rational approach to the purification of corresponding antigenic molecules from toxoplasma.

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