SEQUENTIAL REPLICATION OF BACILLUS SUBTILIS CHROMOSOME, I. COMPARISON OF MARKER FREQUENCIES IN EXPONENTIAL AND STATIONARY GROWTH PHASES

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Abstract
Relative frequencies of various genetic markers in the DNA of B. subtilis, strain W23, in exponential and stationary growth phases were compared using a transformation system. If the chromosome replication has a polarity, the frequency of each marker in the exponential phase should be a function of its location on the chromosome. The results indicate that such polarity exists in B. subtilis. On the basis of the results, a genetic map has been constructed in which the adenine marker is located near the point of origin, from which the chromosome starts replicating, and methionine and isoleucine near the terminus. The results also indicate that chromosomes in the stationary phase are in completed form.