In Vitro Growth and Multiplication of the Malaria Parasite, Plasmodium knowlesi

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Abstract
The erythrocytic form of P. knowlesi was cultivated in vitro, using the "rocker-dilution" and "rocker-perfusion" methods. The red cells were from the monkey, Macaca mulatto. All cultivations were carried on for 24 hrs. under sterile conditions at 38.5[degree] C. The av. increase in parasite count was 3- to 4-fold, which was the same as that for 60 monkeys parasitized in vivo. By the rocker-perfusion method 6 successive generations were obtained. Chem. and metabolic studies were made on parasites grown in vitro and in vivo. The only marked difference was in O2 consumption, this being lower in vitro. The chief growth component furnished by the proteose peptone was probably p-aminobenzoic acid.

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