CHANGE IN GLUCOSAMINE CONTENT OF CHLORELLA CELLS DURING THE COURSE OF THEIR LIFE CYCLE

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Abstract
1. By the VAN WISSELINGH color reaction and the chitosan sulfate test it was revealed that Chlorella cells contain chitosan probably in their cell walls. 2. By fractionating the cell material into several fractions followed by their hydrolysis, it was revealed that the majority of glucosamine was present in the residue material remaining insoluble in ethanol-ether and perchloric acid (PCA) solution. Conceivably, this glucosamine has derived, for the most part, from the chitosan contained in the cell wall material. 3. During the course of life cycle of the algal cells, the increase in content of glucosamine occurred in three steps: first, in proportion to the growth of smaller (young) cells into larger cells; second, corresponding to the formation of autospores within ripened cells; and third, in parallel with the growth of newly born daughter cells. 4. Between the first and second phase mentioned above, there occurred an abrupt breach in the increase of glucosamine. This phenomenon was presumed to be closely related to the profound change in the permeability of cell walls occuring at this transitional stage of cell development.

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