Ligand kinetics of hemoglobin S containing erythrocytes.
- 1 January 1977
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 74 (1) , 203-206
- https://doi.org/10.1073/pnas.74.1.203
Abstract
O2 uptake of fully deoxygenated sickle (SS) erythrocytes is slower than that of normal (AA) erythrocytes, as demonstrated by the half-times of the overall oxygenation reactions: at 25.degree. C in an isotonic phosphate buffer, the normal red cells have a t 1/2 = 82 .+-. 4.7 ms, as compared to sickle red cells where t 1/2 = 135 .+-. 17.6 ms. The effects of temperature, extracellular osmolality and the presence of an antisickling agent (n-butylurea) on the rate of red cell oxygenation strongly suggest that the differences in oxygenation rates encountered with sickle red cells is directly related to the intracellular polymerization of deoxyHb S.This publication has 16 references indexed in Scilit:
- Oxygen transport in sickle cell anemia.1974
- BLOOD OXYGEN DISSOCIATION CURVES IN SICKLE CELL DISEASE1967
- Stopped-flow measurements of CO and O2 uptake by hemoglobin in sheep erythrocytesJournal of Applied Physiology, 1963
- Photocolorimetric Determination of Rate of Uptake of CO and O2 By Reduced Human Red Cell Suspensions at 37°CJournal of Applied Physiology, 1957
- STUDIES ON THE DESTRUCTION OF RED BLOOD CELLS. XII. FACTORS INFLUENCING THE ROLE OF S HEMOGLOBIN IN THE PATHOLOGIC PHYSIOLOGY OF SICKLE CELL ANEMIA AND RELATED DISORDERS 1Journal of Clinical Investigation, 1957
- TITRATABLE SULFHYDRYL GROUPS OF NORMAL AND SICKLE CELL HEMOGLOBINS AT 0-DEGREES AND 38-DEGREES1957
- The kinetics of human haemoglobin in solution and in the red cell at 37° cThe Journal of Physiology, 1955
- OXYGEN DISSOCIATION CURVES IN SICKLE CELL ANEMIA AND IN SUBJECTS WITH THE SICKLE CELL TRAITJournal of Clinical Investigation, 1955
- Some observations on the kinetics of haemoglobin in solution and in the red blood corpuscleBiochemical Journal, 1950
- The rate of distribution of dissolved gases between the red blood corpuscle and its fluid environmentThe Journal of Physiology, 1927