Quantitation of Acetazolamide in Pharmaceutical Dosage Forms Using High-Performance Liquid Chromatography.

Publisher Website
Google Scholar
Abstract
Stability-indicating reverse phase high-pressure liquid chromatography methods to quantify acetazolamide in pharmaceutical dosage forms have been developed. The methods are accurate and precise with percent relative standard deviations based on six injections of 0.9 with a semipolar column and 0.4 with a nonpolar column. No preliminary extraction procedure is required to assay the acetazolamide sodium in vials and a very simple extraction is needed to extract acetazolamide from the tablets or contents of the capsules. Acetazolamide appears to be very unstable in the presence of 0.1N NaOH with a half-life of about 14 days. It underwent hydrolysis to acetic acid and 5-amino-1, 3, 4-thiadiazole-2 sulfonamide. The hydrolysis followed first-order law with a K value of 0.0495 day-1 at 25°.

This publication has 2 references indexed in Scilit: