PURIFICATION AND PARTIAL CHARACTERIZATION OF A MACROPHAGE CYTO-TOXIN FROM PASTEURELLA-HAEMOLYTICA

  • 1 January 1982
    • journal article
    • research article
    • Vol. 43  (5) , 764-767
Abstract
A protein from P. haemolytica that was highly immunogenic and toxic toward bovine alveolar macrophages was partially purified. When isolated from culture supernatants of P. haemolytica serotype 1 or serotype 6, the protein reacted on Ouchterlony immunodiffusion tests with antisera from 12 serotypes of P. haemolytica, but did not cross-react with antisera to serotypes of P. multocida. This indicated that the protein may be specific for P. haemolytica. Bacteria were grown in dialysis culture in a brain-heart infusion and calf-serum growth medium. The protein was isolated from the medium by ultrafiltration and size-exclusion chromatography and has a MW of .apprx. 150,000 daltons. The protein, which is highly immunogenic and has the characteristics of a virulence factor, is common to all serotypes of P. haemolytica, and may be an effective agent for immunization against P. haemolytica in cattle.