AMINOACRIDINE DYES AND THE SECONDARY STRUCTURE OF DNA IN SITU
- 1 December 1971
- journal article
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 19 (12) , 761-765
- https://doi.org/10.1177/19.12.761
Abstract
A new method is proposed to study the secondary structure of deoxyribonucleic acid (DNA) in situ in fixed chromatin. It is based on acriflavine staining and on differentiation with a nitrous acid solution of the fixed cytologic preparation. The presence of green fluorescence after this treatment is regarded as indicative of double stranded DNA. Experiments are described with DNA-acriflavine mixtures in solution, DNA-agar models and cytologic preparations submitted to different pretreatments. The feasibility and limitations of the method are discussed in the light of the results reported upon.Keywords
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