QUANTITATIVE METHOD FOR DETERMINATION OF HEMOLYTIC ACTIVITY OF CLOSTRIDIUM SEPTICUM TOXIN
- 1 January 1987
- journal article
- research article
- Published by Editorial Committee of Japanese Journal of Infectious Diseases, National Institute of Infectious Dis in Japanese Journal of Medical Science and Biology
- Vol. 40 (2) , 47-59
- https://doi.org/10.7883/yoken1952.40.47
Abstract
A quantitative method for titration of hemolytic activity of Clostridium septicum toxin was established. No linear dose-response line was obtained between the toxin dose and hemolysis ratio. Logit or Probit transformation of the hemolysis ratio changed this log-dose response curve into a linear line. We recommend a highly reproducible and accurate method based on parallel line assay using a well-standardized reference toxin for determination of hemolytic activity. The method consists of simultaneous titrations of the hemolysis ratios with each sample and a reference toxin, Logit or Probit transformation of the hemolysis ratio, and expression of the activity in the relative hemolytic value.This publication has 5 references indexed in Scilit:
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