FURTHER-STUDIES ON BIOLOGICAL PROPERTIES OF GUINEA-PIG IGG1 ANTIBODIES .3. HEMOLYTIC EFFICIENCY INVITRO

  • 1 January 1977
    • journal article
    • research article
    • Vol. 32  (1) , 103-109
Abstract
The capacity of non-reciprocally contaminated guinea-pig IgG1 [immunoglobulin G1] and IgG2 antibodies for sensitizing antigen-coated sheep erythrocytes for lysis was studied in the presence of serial dilutions of normal guinea-pig serum as the source of complement. IgG1 antibodies were highly efficient, provided complement was little diluted and not heated at 56.degree.. Chelation of Ca2+ by EGTA [ethylene glycol bis(.beta.-aminoethyl ether) tetraacetic acid] affected lysis of IgG1-sensitized cells by only 50%, while completely blocking lysis of IgG2-sensitized cells. The F(ab'')2 fragment of IgG1, in contrast to the F(ab'')2 fragment of IgG2, was almost as efficient as the parent antibody.