Design and Synthesis of New Reactive Fluorescent Dyes for Cytofluorometry
- 1 January 1982
- journal article
- research article
- Published by Taylor & Francis in Stain Technology
- Vol. 57 (3) , 143-150
- https://doi.org/10.3109/10520298209066606
Abstract
Our purpose has been to synthesize reactive fluorescent compounds with different excitation and/or emission spectra that can be used for multiple fluorescence analysis in combination with the “natural” cell fluorescence exhibited by reduced coenzyme (NAD(P)H) or flavins. Synthesis of condensation products of p-bis(2-chloroethyl)-amino-benzaldehyde is described. Their absorption spectra range from 360 to 480 nm and their emission spectra lie between 520 and 590 nun. Examples of cytological applications are given.This publication has 6 references indexed in Scilit:
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