Application of the peroxidase-antiperoxidase immunoassay to the identification of Salmonellae from pure culture and animal tissue

Publisher Website
Google Scholar
Abstract
The peroxidase-antiperoxidase immunoassay was developed by using selected Salmonella serotypes to evaluate its potential for use in diagnostic bacteriology. S. cholerae-suis var. kunzendorf, S. dublin and S. typhimurium were the test organisms. Strong specific staining with corresponding antiserum was achieved with smears of each Salmonella serotype on microscope slides from formalinized cell suspensions, live broth cultures of clinical isolates and tissue suspensions from the livers and spleens of experimentally infected mice. S. cholerae-suis var. kunzendorf was detected in Formalin-fixed and fresh frozen tissues from experimentally infected pigs. The peroxidase-antiperoxidase assay is thus well-suited for the rapid identification of Salmonella from pure cultures and the technique can be useful for the detection of this pathogen in histological sections.