Purification and Some Properties of Glycyrrhizinic Acid Hydrolase fromAspergillus nigerGRM3
- 1 March 1986
- journal article
- research article
- Published by Oxford University Press (OUP) in Agricultural and Biological Chemistry
- Vol. 50 (3) , 687-692
- https://doi.org/10.1080/00021369.1986.10867443
Abstract
We selected Aspergillus niger GRM3 because it secreted a novel enzyme that hydrolyzed glycyrrhizinic acid. The enzyme was purified to the electrophoretically homogeneous state and an activity more than 890-fold that of culture broth. The molecular weight of the enzyme was estimated to be 150,000 by gel filtration and it was most active over the range of pH 4.1 to 4.5. The enzyme hydrolyzed glycyrrhizinic acid to produce glycyrrhetinic acid and d-glucuronyl-β-1,2-d-glucuronic acid. The hydrolytic action of this enzyme was different from those of β-glucuronidases reported so far.This publication has 0 references indexed in Scilit: