Abstract
Proteins in mammary secretions were examined during the dry period of four cows. Polyacrylamide gel electrophoresis was used to determine relative changes in the electrophoretic profiles of whey proteins in the mammary secretions. Concentrations of .beta.-lactoglobulin and .alpha.-lactalbumin in dry cow secretions were determined by immunoassay. Concentrations of both proteins changed in parallel during the dry period. Concentrations of .beta.-lactoglobulin and .alpha.-lactalbumin decreased during the first 30 d of the dry period but generally remained above .1 mg/ml secretion. A transient increase in .beta.-lactoglobulin and .alpha.-lactalbumin concentrations occurred at about 40 d after drying-off, prior to the prepartum increase.