STRUCTURE OF THE ASPARAGINE-LINKED SUGAR CHAINS OF ALPHA-FETOPROTEIN PURIFIED FROM HUMAN ASCITES-FLUID

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  • 1 January 1980
    • journal article
    • research article
    • Vol. 40  (11) , 4276-4281
Abstract
.alpha.-Fetoprotein purified from human serum contains an asparagine-linked sugar chain in 1 molecule. The sugar chain was quantitatively released from the polypeptide moiety by hydrazinolysis and recovered as oligosaccharides after N-acetylation. The oligosaccharide mixture was separated into a neutral (N) and 2 acidic (A-1 and A-2) fractions by paper electrophoresis. By combination of sequential exoglycosidase digestion, methylation analysis and concanavalin A-sepharose column chromatography, the structures of these fractions were determined. [This information was related to oncofetal antigens secreted from tumors.].