A rapid protocol for sexing chick embryos (Gallus g. domesticus)
- 1 October 1994
- journal article
- Published by Wiley in Animal Genetics
- Vol. 25 (5) , 361-362
- https://doi.org/10.1111/j.1365-2052.1994.tb00374.x
Abstract
Summary: A method for establishing the sex of chick embryos before the appearance or morphological differences between males and females has been developed. DNA was isolated from 5–7‐day‐old embryos by proteinase K digestion and subjected to polymerase chain reaction (PCR) amplification with W‐chromosome specific primers. Sexing can be achieved within 1 day using as little as 1 ng template DNA.Keywords
This publication has 4 references indexed in Scilit:
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- Nucleotide sequences and unusual electrophoretic behavior of the W chromosome-specific repeating DNA units of the domestic fowl, Gallus gallus domesticusChromosoma, 1987
- Complete sequence of the chicken glyceraldehyde-3-phosphate dehydrogenase gene.Proceedings of the National Academy of Sciences, 1985