P2 -purinergic agonists activate phospholipase C in a guanine nucleotide- and Ca2+ -dependent manner in FRTL-5 thyroid cell membranes
- 14 August 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 253 (1-2) , 132-136
- https://doi.org/10.1016/0014-5793(89)80945-7
Abstract
Various adenine nucleotides activated phospholipase C of FRTL-5 cell membranes in the following order of activity, ATPγS > ATP > AppNp > AppCp = ADP > MeSATP. This order was well consistent with that observed in intact cells. Such activation occurred only in the presence of appropriate concentrations of GTPγS and Ca2+, in a way similar to the norepinephrine-induced activation. NaF, a non-specific GTP-binding protein (G-protein) activator, also stimulated the enzyme. These adenine nucleotides, norepinephrine and NaF-induced activations were inhibited by GDPβS. We conclude that a G-protein is involved in the adenine nucleotides-induced activation of phospholipase C via P2-purinergic receptor in FRTL-5 cells.Keywords
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