ActA and human zyxin harbour Arp2/3-independent actin-polymerization activity
- 10 July 2001
- journal article
- research article
- Published by Springer Nature in Nature Cell Biology
- Vol. 3 (8) , 699-707
- https://doi.org/10.1038/35087009
Abstract
The actin cytoskeleton is a dynamic network that is composed of a variety of F-actin structures. To understand how these structures are produced, we tested the capacity of proteins to direct actin polymerization in a bead assay in vitro and in a mitochondrial-targeting assay in cells. We found that human zyxin and the related protein ActA of Listeria monocytogenes can generate new actin structures in a vasodilator-stimulated phosphoprotein-dependent (VASP) manner, but independently of the Arp2/3 complex. These results are consistent with the concept that there are multiple actin-polymerization machines in cells. With these simple tests it is possible to probe the specific function of proteins or identify novel molecules that act upon cellular actin polymerization.Keywords
This publication has 46 references indexed in Scilit:
- Arp2/3 Complex and Actin Depolymerizing Factor/Cofilin in Dendritic Organization and Treadmilling of Actin Filament Array in LamellipodiaThe Journal of cell biology, 1999
- Relationship between Arp2/3 Complex and the Barbed Ends of Actin Filaments at the Leading Edge of Carcinoma Cells after Epidermal Growth Factor StimulationThe Journal of cell biology, 1999
- The Interaction between N-WASP and the Arp2/3 Complex Links Cdc42-Dependent Signals to Actin AssemblyCell, 1999
- Scar, a WASp-related protein, activates nucleation of actin filaments by the Arp2/3 complexProceedings of the National Academy of Sciences, 1999
- The interaction of Arp2/3 complex with actin: Nucleation, high affinity pointed end capping, and formation of branching networks of filamentsProceedings of the National Academy of Sciences, 1998
- Mammalian actin-related protein 2/3 complex localizes to regions of lamellipodial protrusion and is composed of evolutionarily conserved proteinsBiochemical Journal, 1997
- Actin filament organization in the fish keratocyte lamellipodium.The Journal of cell biology, 1995
- L. monocytogenes-induced actin assembly requires the actA gene product, a surface proteinCell, 1992
- Actin filaments and the growth, movement, and spread of the intracellular bacterial parasite, Listeria monocytogenes.The Journal of cell biology, 1989
- Identification of a new protein localized at sites of cell-substrate adhesion.The Journal of cell biology, 1986