A new method of DNA denaturation mapping
- 1 January 1975
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 2 (11) , 2049-2054
- https://doi.org/10.1093/nar/2.11.2049
Abstract
Described is a new method for DNA denaturation mapping utilizing glyoxal (ethanedial) to stabilize the denatured regions. The extent of glyoxal reaction can be easily and sensitively measured using an assay based on the intercalation of ethidium into duplex DNA. Thus denturation maps can be produced in a controlled way under a wide variety of conditions.Keywords
This publication has 8 references indexed in Scilit:
- A new method for mapping nucleic acid sequence homology by electron microscopyNucleic Acids Research, 1975
- Native and denatured DNA, cross-linked and palindromic DNA and circular covalently-closed DNA analysed by a sensitive fluorometric procedureBiochemical and Biophysical Research Communications, 1974
- An Electron Microscope Study of Sindbis Virus RNACold Spring Harbor Symposia on Quantitative Biology, 1974
- A Fluorescence Assay for DNA with Covalently Linked Complementary SequencesCanadian Journal of Biochemistry, 1972
- The reaction of glyoxal with nucleic acid components III. Kinetics of the reaction with monomersBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1971
- Denaturation maps of the left and right sides of the lambda DNA molecule determined by electron microscopyJournal of Molecular Biology, 1967
- The Reaction of Guanine Derivatives with 1,2-Dicarbonyl Compounds*Biochemistry, 1966
- A denaturation map of the λ phage DNA molecule determined by electron microscopyJournal of Molecular Biology, 1966