Factors Affecting Aflatoxin Production by Aspergillus parasiticus in a Chemically Defined Medium

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Abstract
The optimum levels of sucrose, (NH4)2SO4, MgSO4, KH2PO4 and ZnSO4 for aflatoxin production in a chemically defined medium were established. The last 2 were essential for fungal growth and aflatoxin production. The effect of various C sources on alfatoxin production was tested using the defined medium. Asparagine was essential for aflatoxin production. Very little aflatoxin was produced in the absence of asparagine with any of the other inorganic N sources tested. Supplementation with yeast extract, Casamino acids, Casitone and peptone increased the aflatoxin yield, but omission of asparagine led to decreased aflatoxin yields even when complex N sources were present. Asparagine could be replaced by aspartic acid or alanine.