Separation and Identification of Lecithins from Hevea Latex

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Abstract
To identify completely the phospholipids, which according to several authors may occur in Hevea latex, we separated in a very simple way a butter-like substance, which really contains phospholipids. The results of qualitative analysis justify the positive conclusion that this substance contains lecithins composed of glycerophosphoric acid, choline and the fat acids: palmitic acid, stearic acid, arachidic acid, oleic acid and linoleic acid. A glycoside also was shown to be present in the raw material. We found the content of phosphorus, nitrogen and fat acids to be 1.58, 0.54 and 84.1 per cent, respectively. In purifying the crude material by means of fractional precipitation with acetone from the ether solution, we obtained a nearly colorless gum which contained 2.35 per cent phosphorus, 1.05 per cent nitrogen and 61.3 per cent fat acids. It is stated that the purification is accompanied by a considerable loss of fat acids, the latter remaining in the acetone-ether solution.

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