CURRENT CLAMP AND VOLTAGE CLAMP STUDY OF INHIBITORY ACTION OF DNP ON MEMBRANE ELECTRICAL-PROPERTIES OF FROG AURICULAR HEART-MUSCLE

  • 1 January 1976
    • journal article
    • research article
    • Vol. 72  (2) , 171-180
Abstract
Current clamp studies showed that after 10 min under DNP [dinitrophenol] [10-4 M] the membrane potential did not change significantly while an important shortening of the action potential duration and a diminished amplitude were observed. Voltage clamp studies were performed on the slow inward and delayed outward currents. DNP (10-4 M) induced a marked decrease of the slow inward current related to the reduction in both conductance and driving force, and a decrease in the amplitude of the delayed current. The decrease of the slow inward current seemed to be mainly responsible for the suppression of the plateau of the action potential during metabolic inhibition.