PHARMACOLOGIC AND TOXICOLOGIC EVALUATION OF THIOPROLINE - A PROPOSED NONTOXIC INDUCER OF REVERSE TRANSFORMATION

  • 1 January 1980
    • journal article
    • research article
    • Vol. 64  (8-9) , 837-844
Abstract
The ability of thioproline to act as a nontoxic inducer of reverse transformation was examined. Thioproline was unable to effect reproducibly morphologic changes characteristic of the reversed transformed state in HeLa [human cervical carcinoma], WI-38VA13 [human lung neoplastic], C1300 neuroblastoma [mouse] and CHO-K1 [Chinese hamster ovary] cell lines. Reversal of transformed cells with dibutyryl cAMP was readily demonstrated in CHO-K1 and C1300 cell lines. Thioproline produced severe, life-threatening CNS toxicity in mice and rats at levels far below those previously reported to be without any toxic effects. Thioproline is incapable of producing a reversal of the transformed state in HeLa and other cell lines in vitro and is a compound that must be described as a strongly CNS toxic agent.