THE DETERMINATION OF 17-KETOGENIC STEROIDS IN URINE

Publisher Website
Google Scholar
Abstract
Urine was treated with sodium borohydride to reduce existing 17-ketosteroids (17-KS), thus eliminating the need of a preliminary 17-KS determination. This procedure also reduced the di-hydroxy acetone side chain of cortisone metabolites to a glycerol form. The steroids were then liberated from their conjugates by boiling with hot dilute mineral acid. The cooled acidic solution was then treated with potassium periodate at room temperature for 2 hours. The 17-KS formed by this procedure were then extracted and measured. After oral administration of cortisone acetate, approximately 40% of the dose was recovered in the urine during the first 24 hours.