Equilibrium melting of plasmid ColE1 DNA: electron-microscopic visualization
Open Access
- 25 September 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 8 (18) , 4165-4184
- https://doi.org/10.1093/nar/8.18.4165
Abstract
The fine structure of the melting curve for the linear ColE1 DNA has been obtained. To find the ColE1 DNA regions corresponding to peaks in the melting curve's fine structure, we fixed the melted DNA regions with glyoxal /12/. Electron-microscopic denaturation maps were obtained for nine temperature points within the melting range. Thereby the whole process of ColE1 DNA melting was reconstructed in detail. Spectrophotometric and electron microscopic data were used for mapping the distribution of GC-pairs over the DNA. molecule. The most AT-rich DNA regions (28 and 37% of GC-pairs), 380 and 660 bp long resp., are located on both sides of the site of ColE1 DNA's cleavage by EcoR1 endonuclease. The equilibrium denaturation maps are compared with maps obtained by the method of Inman /20/ for eight points of the kinetic curve of ColE1 DNA unwinding by formaldehyde.Keywords
This publication has 15 references indexed in Scilit:
- Location of the cooperative melting regions in bacteriophage fd DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1978
- A rapid procedure for purification of EcoRI endonucleaseBiochemical and Biophysical Research Communications, 1977
- Specific fragmentation of T7 phage DNA at low-melting sitesNucleic Acids Research, 1977
- Fine structure of DNA melting curvesBiopolymers, 1976
- High resolution thermal denaturation of DNA: thermalites of bacteriophage DNABiochemistry, 1976
- Fine structures in denaturation curves of bacteriophage lambda DNA. Their relation to the intramolecular heterogeneity in base compositionBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1975
- Base composition heterogeneity in kinetoplast DNA from four species of hemoflagellatesBiochemical and Biophysical Research Communications, 1974
- Hysteresis and partial irreversibility of denaturation of DNA as a means of investigating the topology of base distribution constraints: Application to a yeast ρ− (Petite) mitochondrial DNAJournal of Molecular Biology, 1974
- The reaction of glyoxal with nucleic acid components: V. Denaturation of DNA under the action of glyoxalBiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Denaturation map of bacteriophage T7 DNA and direction of DNA transcriptionJournal of Molecular Biology, 1972