Polymerase chain reaction (PCR) in the quality and safety assurance of food: Detection of soya in processed meat products
- 1 July 1996
- journal article
- Published by Springer Nature in Zeitschrift für Lebensmittel-Untersuchung und Forschung
- Vol. 203 (4) , 339-344
- https://doi.org/10.1007/bf01231072
Abstract
A new method for the specific and sensitive detection of soya (Glycine max) in processed meat products has been developed using polymerase chain reaction (PCR) technology. The presence of soya deoxyribonucleic acid (DNA) from several soya protein concentrates was determined with two pairs of specific oligonucleotides yielding a 414-bp (bp=base pair) fragment and an internal 118-bp fragment amplified from the soya lectinLe1 gene. The test detected DNA from textured soya protein concentrates in meat products at a level of 1% and was confirmed by a commercially available enzyme-linked immunosorbent assay (ELISA).Keywords
This publication has 5 references indexed in Scilit:
- Nachweis gentechnologisch ver nderter Pflanzen mittels der Polymerase Kettenreaktion (PCR) am Beispiel derFlavr Savr TM-TomateZeitschrift für Lebensmittel-Untersuchung und Forschung, 1995
- Polymerase chain reaction (PCR): a possible alternative to immunochemical methods assuring safety and quality of food Detection of wheat contamination in non-wheat food productsZeitschrift für Lebensmittel-Untersuchung und Forschung, 1993
- An assessment of commercially available reagents for an enzyme‐linked immunosorbent assay (ELISA) of soya protein in meat productsJournal of the Science of Food and Agriculture, 1984
- Ca lectin gene insertion has the structural features of a transposable elementCell, 1983
- Determination of soya proteins in food using an enzyme-linked immunosorbent assay procedureJournal of the Science of Food and Agriculture, 1981