Characterization of a new picorna-like virus, himetobi P virus, in planthoppers

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Abstract
Picorna-like virus particles, 29 nm in diameter, were purified from apparently healthy Laodelphax striatellus Fallen. The virus particles had a buoyant density of 1.352 g/ml in CsCl and a sedimentation coefficient of 161 s. The virus capsid proteins consisted of three major polypeptides of M rs 36500, 33000 and 28000, and three minor polypeptides. The virus contained a major ssRNA of M r 2.8 × 106 and was also frequently associated with a minor dsRNA of M r 4×106. The 3′ end of the ssRNA had a poly(A) tract of about 60 adenine residues. The virus has been provisionally named himetobi P virus.

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