CHARACTERIZATION OF N-GLYCOLYLNEURAMINIC ACID-CONTAINING GANGLIOSIDES AS TUMOR-ASSOCIATED HANGANUTZIU-DEICHER ANTIGEN IN HUMAN-COLON CANCER

  • 1 January 1985
    • journal article
    • research article
    • Vol. 45  (8) , 3796-3802
Abstract
Hanganutziu-Deicher (HD) antigen-active N-glycolylneuraminic acid (NeuGc)-containing gangliosides were isolated and characterized from human colon cancer tissues. The antigenic gangliosides were detected by TLC by a newly developed method (Higashi et al. 1984) of enzyme immunostaining using affinity-purified chicken antibody against hematoside containing NeuGc (II3NeuGc-LacCer) and horseradish peroxidase-conjugated rabbit anti-chicken IgG. One to 6 species of the antigenic gangliosides were isolated from 7 of 16 cases of colon cancer, whereas no antigenic compound was detected in all apparently normal colorectal tissues from 17 individuals without colorectal cancer. Tissues from different patients showed different patterns of molecular species of the antigenic gangliosides. Densitometric determination indicated that HD antigenic sialic acid, NeuGc, accounted for about 1% or less of the total lipid-bound sialic acids. Four species of antigenic gangliosides were identified as hematoside and hematoside-containing O-acyl ester (II3NeuGc-LacCer and II34- or 7-O-acyl-NeuGc-LacCer), GM2-containing NeuGc (II3NeuGc-GgOse3Cer), and sialylparagloboside (IV3NeuGc-nLcOse4Cer) by their behaviors on 2-dimensional TLC and the effects of mild alkaline treatment, sialidase treatment, periodate oxidation, and endo-.beta.-galactosidase treatment.

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