Properties of Amino Acid Composition of the Tryptic Fragments of the Heavy Chain of Myosin Subfragment-1
- 1 July 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in The Journal of Biochemistry
- Vol. 90 (3) , 785-788
- https://doi.org/10.1093/oxfordjournals.jbchem.a133534
Abstract
Limited tryptic digestion of the heavy chain of chymotryptic myosin subfragment-1 gives three major fragments with approximate molecular weights of 50 K, 27 K, and 20 K daltons. We previously reported (Hozumi, T. & Muhlrad, A. (1981) Biochemistry20, 2945–2950) that these fragments can be separated by gel filtration in 1% sodium dodecyl sulfate. By using this method, the tryptophan content and amino acid composition of fragments were measured. The 27 K and 50 K fragments were found to be rich in tryptophan, in contrast to the 20 K fragment.Keywords
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