Productions and Utilizations ofβ-Amylase and Pullulanase fromBacillus cereusvar.mycoides

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Abstract
A bacterium which produces both a β-amylase and a pullulanase effective for producing maltose from starch was isolated from soil. The strain was identified to be Bacillus cereus var. mycoides. The optimum conditions for converting starch to maltose with the enzymes were pH 6–6.5 and about 50°C. The yield of maltose from starch depended on the degree of liquefaction of starch, and the maximum yield of maltose from starch was about 95%. The culture conditions of the strain for the simultaneous production of the enzymes were also investigated.