Negative regulation of human insulin gene expression by the 5′‐flanking region in non‐pancreatic cells
- 10 April 1989
- journal article
- Published by Wiley in FEBS Letters
- Vol. 247 (1) , 41-45
- https://doi.org/10.1016/0014-5793(89)81236-0
Abstract
We have examined the effect of the 5′-flanking region of the human insulin gene on its expression in non-pancreatic cells. The presence of the region containing the insulin gene enhancer (−339 to −169 bp) markedly repressed the promoter activity of the insulin gene. This suppressive phenomenon was restored by the addition of forskolin or dibutyryl cAMP, suggesting that this region alone is not sufficient to repress completely insulin gene expression in the presence of extracellular stimuli which increase the intracellular cAMP level. The hypervariable region (HVR) located at −365 bp also repressed the promoter activity. These results show negative regulation of human insulin gene expression in non-pancreatic cells by these regions.Keywords
This publication has 24 references indexed in Scilit:
- Modulation of Insulin Secretion From β-Cells by Phosphoinositide-Derived Second-Messenger MoleculesDiabetes, 1988
- A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genesNature, 1986
- STRUCTURE AND EVOLUTION OF THE INSULIN GENEAnnual Review of Genetics, 1985
- Cell-Specific Expression of the Rat Insulin Gene: Evidence for Role of Two Distinct 5′ Flanking ElementsScience, 1985
- Sequence and Structure of a Human Glucose TransporterScience, 1985
- An exonuclease protection assay reveals heat-shock element and TATA box DNA-binding proteins in crude nuclear extractsNature, 1985
- Heritable formation of pancreatic β-cell tumours in transgenic mice expressing recombinant insulin/simian virus 40 oncogenesNature, 1985
- Cell-specific expression controlled by the 5′-flanking region of insulin and chymotrypsin genesNature, 1983
- The highly polymorphic region near the human insulin gene is composed of simple tandemly repeating sequencesNature, 1982
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973